Zachodniopomorski Uniwersytet Technologiczny w Szczecinie

Wydział Biotechnologii i Hodowli Zwierząt - Biotechnology (S2)

Sylabus przedmiotu Enzyme engineering:

Informacje podstawowe

Kierunek studiów Biotechnology
Forma studiów studia stacjonarne Poziom drugiego stopnia
Tytuł zawodowy absolwenta magister inżynier
Obszary studiów charakterystyki PRK, kompetencje inżynierskie PRK
Profil ogólnoakademicki
Moduł
Przedmiot Enzyme engineering
Specjalność Biotechnology in animal production and environmental protection
Jednostka prowadząca Katedra Mikrobiologii i Biotechnologii
Nauczyciel odpowiedzialny Radosław Drozd <Radoslaw.Drozd@zut.edu.pl>
Inni nauczyciele
ECTS (planowane) 4,0 ECTS (formy) 4,0
Forma zaliczenia egzamin Język angielski
Blok obieralny Grupa obieralna

Formy dydaktyczne

Forma dydaktycznaKODSemestrGodzinyECTSWagaZaliczenie
laboratoriaL3 15 1,00,29zaliczenie
ćwiczenia audytoryjneA3 15 1,00,29zaliczenie
wykładyW3 15 2,00,42egzamin

Wymagania wstępne

KODWymaganie wstępne
W-1The basic knowledge of organic and inorganic chemistry, biochemistry, biophysics, physical chemistry, English at intermediate level

Cele przedmiotu

KODCel modułu/przedmiotu
C-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization

Treści programowe z podziałem na formy zajęć

KODTreść programowaGodziny
ćwiczenia audytoryjne
T-A-1The industrial application of the enzymes from the oxidoreductase classes2
T-A-2The industrial application of the enzymes from the transferase classes2
T-A-3The industrial application of the enzymes from the hydrolase classes2
T-A-4The industrial application of the enzymes from the lyase classes2
T-A-5The industrial application of the enzymes from the isomerase classes2
T-A-6The industrial application of the enzymes from the ligase classe2
T-A-7Optimization of the properties of enzymes for use in industrial processes3
15
laboratoria
T-L-1Methods of visualization of protein structures with using the specialized software for molecular modeling2
T-L-2The computational methods for protein structure determination on the their second and third level organizationji4
T-L-3Modeling the catalytic properties of the enzymes2
T-L-4Methods of the enzymes isolation and purification2
T-L-5Methods for the immobilization of enzymes on the organic and inorganic matrices5
15
wykłady
T-W-1The function and significance of the structure of enzymes. The impact amino acid molecular interaction on the stabilization the structure of enzymatic proteins. Methods for the analysis of the primary structure of the enzyme proteins. Methods for the analysis of secondary and tertiary level structure of the enzyme proteins2
T-W-2The methods of exploring structural and sequence protein databases. The specialist molecular modeling software; practical approach to the comparison of the structure I, II and III the order of the enzymes. Methods of solving the 3D structure of enzymes. Classical methods (X-ray, NMR) vs. comparative modeling and ab-initio methods2
T-W-3Catalytic properties of enzymes. Methods of the in-vitro study of the enzymes active site properities2
T-W-4Modeling and optimization of kinetic parameters of enzymes. Prediction and design the catalytic properties of enzymes by in-silico methods.2
T-W-5The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources2
T-W-6Microbial production of enzymes with different properties. Types of fermentation processes. Design and optimization of bioreactors for the production of the enzymes on an industrial scale2
T-W-7The enzymes in food and pharmaceutical industries. The enzymes in the textile and material2
T-W-8The application of enzymes in environmental remediation and fuel production from renewable sources. he application of enzyme in the household1
15

Obciążenie pracą studenta - formy aktywności

KODForma aktywnościGodziny
ćwiczenia audytoryjne
A-A-1participation in classes15
A-A-2preparation for classes5
A-A-3reading the indicated literature10
30
laboratoria
A-L-1participation in classes15
A-L-2preparation for laboratory classes10
A-L-3reading the indicated literature5
30
wykłady
A-W-1preparation for course passing15
A-W-2participation in classes15
A-W-3reading the indicated literature5
A-W-4preparation for classes10
A-W-5contact hours15
60

Metody nauczania / narzędzia dydaktyczne

KODMetoda nauczania / narzędzie dydaktyczne
M-1information lecture
M-2narrative lectures
M-3anecdote
M-4conversational lecture

Sposoby oceny

KODSposób oceny
S-1Ocena podsumowująca: Written exam, test
S-2Ocena formująca: Report from laboratory classes Multimedia presentation

Zamierzone efekty uczenia się - wiedza

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
BT_2A_BTZ-A-D8_W01
Student has a knowledge of the scope of structural dependencies shaping the catalytic properties of enzymes. Student knows bioinforamtics tools that allow prediction of the enzyme structure.
BTap_2A_W07C-1, C-2, C-3T-L-2, T-L-3M-2, M-3, M-4, M-1S-1, S-2
BT_2A_BTZ-A-D8_W02
Student knows the methods of enzyme immobilization and methods preparation of highly purified enzyme preparations.
BTap_2A_W07C-1, C-2, C-3T-L-4, T-L-5, T-W-1, T-W-5, T-W-4M-2, M-3, M-4, M-1S-1, S-2
BT_2A_BTZ-A-D8_W03
Student knows the methods of selection of microorganisms for application tasks aimed at the production of species with specific catalytic activity
BTap_2A_W07C-1, C-2, C-3T-L-5, T-A-5, T-A-1, T-A-2, T-A-3, T-A-4, T-A-6, T-A-7, T-W-5, T-W-6, T-W-8M-2, M-3, M-4, M-1S-1, S-2

Zamierzone efekty uczenia się - umiejętności

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
BT_2A_BTZ-A-D8_U01
Student use bioinformatic tools to solve the enzyme structure, is able to gain dependence between their structure and catalytic functions
BTap_2A_U07C-1, C-2, C-3T-L-2, T-L-1, T-L-3, T-W-5, T-W-4M-2, M-3, M-4, M-1S-2
BT_2A_BTZ-A-D8_U02
Student analyze methods that allow enzyme immoblizations, calculates the possibilities of their application in specific applications
BTap_2A_U07C-1, C-2, C-3T-L-5, T-A-7, T-W-5, T-W-6, T-W-7, T-W-4M-2, M-3, M-4, M-1S-1, S-2

Zamierzone efekty uczenia się - inne kompetencje społeczne i personalne

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
BT_2A_BTZ-A-D8_K01
Student knows and uses biotechnology methods to improve the quality of life for himself and others
BTap_2A_K02, BTap_2A_K03, BTap_2A_K04C-1, C-2, C-3T-A-7, T-W-8M-2, M-3, M-4, M-1S-1, S-2

Kryterium oceny - wiedza

Efekt uczenia sięOcenaKryterium oceny
BT_2A_BTZ-A-D8_W01
Student has a knowledge of the scope of structural dependencies shaping the catalytic properties of enzymes. Student knows bioinforamtics tools that allow prediction of the enzyme structure.
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
BT_2A_BTZ-A-D8_W02
Student knows the methods of enzyme immobilization and methods preparation of highly purified enzyme preparations.
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
BT_2A_BTZ-A-D8_W03
Student knows the methods of selection of microorganisms for application tasks aimed at the production of species with specific catalytic activity
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0

Kryterium oceny - umiejętności

Efekt uczenia sięOcenaKryterium oceny
BT_2A_BTZ-A-D8_U01
Student use bioinformatic tools to solve the enzyme structure, is able to gain dependence between their structure and catalytic functions
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
BT_2A_BTZ-A-D8_U02
Student analyze methods that allow enzyme immoblizations, calculates the possibilities of their application in specific applications
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0

Kryterium oceny - inne kompetencje społeczne i personalne

Efekt uczenia sięOcenaKryterium oceny
BT_2A_BTZ-A-D8_K01
Student knows and uses biotechnology methods to improve the quality of life for himself and others
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0

Literatura podstawowa

  1. Yoo, Y.J., Feng, Y., Kim, Y.-H., Yagonia, C., Fundamentals of Enzyme Engineering, Springer Netherlands, 2017
  2. Wolfgang Aehle red., Enzymes in Industry: Production and Applications, Willey VCH, 2007, III
  3. Allan Svendsen, Enzyme Functionality: Design, Engineering and Screening, 2004
  4. Christoph Wittmann i Rainer Krull red., Biosystems Engineering I: Creating Superior Biocatalysts, Tom 1, Springer, 2010
  5. Girish Shukla i Ajit Varma, Soil Enzymology, Springer, 2011

Literatura dodatkowa

  1. Athel Cornish-Bowden, Fundamentals of Enzyme Kinetics, Portland Press, Londyn, 2002, III

Treści programowe - ćwiczenia audytoryjne

KODTreść programowaGodziny
T-A-1The industrial application of the enzymes from the oxidoreductase classes2
T-A-2The industrial application of the enzymes from the transferase classes2
T-A-3The industrial application of the enzymes from the hydrolase classes2
T-A-4The industrial application of the enzymes from the lyase classes2
T-A-5The industrial application of the enzymes from the isomerase classes2
T-A-6The industrial application of the enzymes from the ligase classe2
T-A-7Optimization of the properties of enzymes for use in industrial processes3
15

Treści programowe - laboratoria

KODTreść programowaGodziny
T-L-1Methods of visualization of protein structures with using the specialized software for molecular modeling2
T-L-2The computational methods for protein structure determination on the their second and third level organizationji4
T-L-3Modeling the catalytic properties of the enzymes2
T-L-4Methods of the enzymes isolation and purification2
T-L-5Methods for the immobilization of enzymes on the organic and inorganic matrices5
15

Treści programowe - wykłady

KODTreść programowaGodziny
T-W-1The function and significance of the structure of enzymes. The impact amino acid molecular interaction on the stabilization the structure of enzymatic proteins. Methods for the analysis of the primary structure of the enzyme proteins. Methods for the analysis of secondary and tertiary level structure of the enzyme proteins2
T-W-2The methods of exploring structural and sequence protein databases. The specialist molecular modeling software; practical approach to the comparison of the structure I, II and III the order of the enzymes. Methods of solving the 3D structure of enzymes. Classical methods (X-ray, NMR) vs. comparative modeling and ab-initio methods2
T-W-3Catalytic properties of enzymes. Methods of the in-vitro study of the enzymes active site properities2
T-W-4Modeling and optimization of kinetic parameters of enzymes. Prediction and design the catalytic properties of enzymes by in-silico methods.2
T-W-5The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources2
T-W-6Microbial production of enzymes with different properties. Types of fermentation processes. Design and optimization of bioreactors for the production of the enzymes on an industrial scale2
T-W-7The enzymes in food and pharmaceutical industries. The enzymes in the textile and material2
T-W-8The application of enzymes in environmental remediation and fuel production from renewable sources. he application of enzyme in the household1
15

Formy aktywności - ćwiczenia audytoryjne

KODForma aktywnościGodziny
A-A-1participation in classes15
A-A-2preparation for classes5
A-A-3reading the indicated literature10
30
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta

Formy aktywności - laboratoria

KODForma aktywnościGodziny
A-L-1participation in classes15
A-L-2preparation for laboratory classes10
A-L-3reading the indicated literature5
30
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta

Formy aktywności - wykłady

KODForma aktywnościGodziny
A-W-1preparation for course passing15
A-W-2participation in classes15
A-W-3reading the indicated literature5
A-W-4preparation for classes10
A-W-5contact hours15
60
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBT_2A_BTZ-A-D8_W01Student has a knowledge of the scope of structural dependencies shaping the catalytic properties of enzymes. Student knows bioinforamtics tools that allow prediction of the enzyme structure.
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_W07has an extended knowledge of engineering processes, devices and technological lines used in biotechnology
Cel przedmiotuC-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization
Treści programoweT-L-2The computational methods for protein structure determination on the their second and third level organizationji
T-L-3Modeling the catalytic properties of the enzymes
Metody nauczaniaM-2narrative lectures
M-3anecdote
M-4conversational lecture
M-1information lecture
Sposób ocenyS-1Ocena podsumowująca: Written exam, test
S-2Ocena formująca: Report from laboratory classes Multimedia presentation
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBT_2A_BTZ-A-D8_W02Student knows the methods of enzyme immobilization and methods preparation of highly purified enzyme preparations.
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_W07has an extended knowledge of engineering processes, devices and technological lines used in biotechnology
Cel przedmiotuC-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization
Treści programoweT-L-4Methods of the enzymes isolation and purification
T-L-5Methods for the immobilization of enzymes on the organic and inorganic matrices
T-W-1The function and significance of the structure of enzymes. The impact amino acid molecular interaction on the stabilization the structure of enzymatic proteins. Methods for the analysis of the primary structure of the enzyme proteins. Methods for the analysis of secondary and tertiary level structure of the enzyme proteins
T-W-5The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources
T-W-4Modeling and optimization of kinetic parameters of enzymes. Prediction and design the catalytic properties of enzymes by in-silico methods.
Metody nauczaniaM-2narrative lectures
M-3anecdote
M-4conversational lecture
M-1information lecture
Sposób ocenyS-1Ocena podsumowująca: Written exam, test
S-2Ocena formująca: Report from laboratory classes Multimedia presentation
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBT_2A_BTZ-A-D8_W03Student knows the methods of selection of microorganisms for application tasks aimed at the production of species with specific catalytic activity
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_W07has an extended knowledge of engineering processes, devices and technological lines used in biotechnology
Cel przedmiotuC-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization
Treści programoweT-L-5Methods for the immobilization of enzymes on the organic and inorganic matrices
T-A-5The industrial application of the enzymes from the isomerase classes
T-A-1The industrial application of the enzymes from the oxidoreductase classes
T-A-2The industrial application of the enzymes from the transferase classes
T-A-3The industrial application of the enzymes from the hydrolase classes
T-A-4The industrial application of the enzymes from the lyase classes
T-A-6The industrial application of the enzymes from the ligase classe
T-A-7Optimization of the properties of enzymes for use in industrial processes
T-W-5The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources
T-W-6Microbial production of enzymes with different properties. Types of fermentation processes. Design and optimization of bioreactors for the production of the enzymes on an industrial scale
T-W-8The application of enzymes in environmental remediation and fuel production from renewable sources. he application of enzyme in the household
Metody nauczaniaM-2narrative lectures
M-3anecdote
M-4conversational lecture
M-1information lecture
Sposób ocenyS-1Ocena podsumowująca: Written exam, test
S-2Ocena formująca: Report from laboratory classes Multimedia presentation
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBT_2A_BTZ-A-D8_U01Student use bioinformatic tools to solve the enzyme structure, is able to gain dependence between their structure and catalytic functions
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_U07analyzes the main metabolic pathways and mechanisms for their regulation based on knowledge of the structure and function of proteins, hormones and vitamins; can acquire and use enzymes
Cel przedmiotuC-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization
Treści programoweT-L-2The computational methods for protein structure determination on the their second and third level organizationji
T-L-1Methods of visualization of protein structures with using the specialized software for molecular modeling
T-L-3Modeling the catalytic properties of the enzymes
T-W-5The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources
T-W-4Modeling and optimization of kinetic parameters of enzymes. Prediction and design the catalytic properties of enzymes by in-silico methods.
Metody nauczaniaM-2narrative lectures
M-3anecdote
M-4conversational lecture
M-1information lecture
Sposób ocenyS-2Ocena formująca: Report from laboratory classes Multimedia presentation
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBT_2A_BTZ-A-D8_U02Student analyze methods that allow enzyme immoblizations, calculates the possibilities of their application in specific applications
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_U07analyzes the main metabolic pathways and mechanisms for their regulation based on knowledge of the structure and function of proteins, hormones and vitamins; can acquire and use enzymes
Cel przedmiotuC-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization
Treści programoweT-L-5Methods for the immobilization of enzymes on the organic and inorganic matrices
T-A-7Optimization of the properties of enzymes for use in industrial processes
T-W-5The methods of the immobilisation and stabilisation the enzymes. The purification and isolation of the enzyme from different sources
T-W-6Microbial production of enzymes with different properties. Types of fermentation processes. Design and optimization of bioreactors for the production of the enzymes on an industrial scale
T-W-7The enzymes in food and pharmaceutical industries. The enzymes in the textile and material
T-W-4Modeling and optimization of kinetic parameters of enzymes. Prediction and design the catalytic properties of enzymes by in-silico methods.
Metody nauczaniaM-2narrative lectures
M-3anecdote
M-4conversational lecture
M-1information lecture
Sposób ocenyS-1Ocena podsumowująca: Written exam, test
S-2Ocena formująca: Report from laboratory classes Multimedia presentation
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBT_2A_BTZ-A-D8_K01Student knows and uses biotechnology methods to improve the quality of life for himself and others
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_K02demonstrates understanding of biotechnological processes used in various areas of human activity; interprets and describes these processes using a scientific approach
BTap_2A_K03is aware of the impact of biotechnology on the shaping and condition of the natural environment and human health
BTap_2A_K04is aware of the ethical and social standards related to the carried out research and professional activities; understands the desirability of proceeding in accordance with the established ethical and legal principles
Cel przedmiotuC-1Familiarize the students with the basics methods of the proteins engineering for modifying the catalytic activity of enzymes
C-2Introduction to methods of in silico prediction of protein structures
C-3Mastering the skills of the selection of appropriate methods for enzyme immobilization
Treści programoweT-A-7Optimization of the properties of enzymes for use in industrial processes
T-W-8The application of enzymes in environmental remediation and fuel production from renewable sources. he application of enzyme in the household
Metody nauczaniaM-2narrative lectures
M-3anecdote
M-4conversational lecture
M-1information lecture
Sposób ocenyS-1Ocena podsumowująca: Written exam, test
S-2Ocena formująca: Report from laboratory classes Multimedia presentation
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Preparation of a multimedia presentation, participation in 90% of auditory classes, Correct answer to 60% of test questions - exam
3,5
4,0
4,5
5,0