Zachodniopomorski Uniwersytet Technologiczny w Szczecinie

Wydział Biotechnologii i Hodowli Zwierząt - Biotechnology (S2)

Sylabus przedmiotu Basics of contemporary microscopy:

Informacje podstawowe

Kierunek studiów Biotechnology
Forma studiów studia stacjonarne Poziom drugiego stopnia
Tytuł zawodowy absolwenta magister inżynier
Obszary studiów charakterystyki PRK, kompetencje inżynierskie PRK
Profil ogólnoakademicki
Moduł
Przedmiot Basics of contemporary microscopy
Specjalność przedmiot wspólny
Jednostka prowadząca Katedra Fizjologii, Cytobiologii i Proteomiki
Nauczyciel odpowiedzialny Katarzyna Michałek <Katarzyna.Michalek@zut.edu.pl>
Inni nauczyciele Agnieszka Kochmańska <Agnieszka.Kochmanska@zut.edu.pl>
ECTS (planowane) 4,0 ECTS (formy) 4,0
Forma zaliczenia egzamin Język angielski
Blok obieralny Grupa obieralna

Formy dydaktyczne

Forma dydaktycznaKODSemestrGodzinyECTSWagaZaliczenie
wykładyW1 20 2,00,59egzamin
laboratoriaL1 20 2,00,41zaliczenie

Wymagania wstępne

KODWymaganie wstępne
W-1Basics of the physics and structure, morphology of living organisms

Cele przedmiotu

KODCel modułu/przedmiotu
C-1Get knowledge about modern microscopy
C-2To familiarise students with the microscopy techniques and equipment used in the study of living organisms structure

Treści programowe z podziałem na formy zajęć

KODTreść programowaGodziny
laboratoria
T-L-1Drying at a critical point of CO2, vacuum spraying of samples using in SEM, observations in SEM, interpretation of SE and BSE images.3
T-L-2Optical microscopy. Metallographic and biologic microscope. Observations in the light and dark field; in polarized light and using interference contrast.3
T-L-3X-ray EDS microanalysis.2
T-L-4Atomic force microscope AFM2
T-L-5The hematoxylin and eosine procedures (H&E). Application of H&E stain. Staining of tissue section with the use of H&E technique.3
T-L-6Histochemical techniques of tissue staining. Application of periodic acid Schiff (PAS) stain. Staining of renal tissue wit the use of PAS technique.3
T-L-7Immunohistechemistry (IHC). Application of IHC. Antigen retrieval. Permabilization. Blocking. Primary antibody selection and optimization. Antibody specifity. Chromogenic detection. IHC controls. Immunolocalization of aquaporin 1 (AQP1) in the renal cortex and medulla4
20
wykłady
T-W-1Basics of light optics. Construction of an optical microscope. Types of microscopic techniques in modern biology.3
T-W-2Basics of electron optics.1
T-W-3Scanning electron microscope SEM. Principle of working, construction of the microscope. Types of images. Preparation.3
T-W-4Transmission electron microscope TEM. Principle of working, construction of the microscope. Preparation.3
T-W-5X-ray microanalysis EDS and WDS.2
T-W-6Scanning Probe Microscope SPM: Scanning Tunelling Microscope STM; Atomic Force Microscope AFM.2
T-W-7Collection and preparation of biological samples for microscopic examination. Fixation, dehydratation, embedding and sectioning. Tissue preservation. Parraffin-embedded tissue. Frozen tissue. Microtome. Crystostat.2
T-W-8Tissue staining. Histological techniques (hematoxylin and eosin (H&E), periodic acid Schiff (PAS), Masson’s trichome stain, van Gison’s stain. Immunohistochemistry application. Analysis and interpretation of microscopic images4
20

Obciążenie pracą studenta - formy aktywności

KODForma aktywnościGodziny
laboratoria
A-L-1Mandatory participation in laboratories20
A-L-2Study of recommended literature10
A-L-3Preparing for the laboratory analysis20
A-L-4Consultation with the teacher10
60
wykłady
A-W-1Participation in lectures20
A-W-2Learning for the colloquium10
A-W-3Consultation with the teacher10
A-W-4Self study content of the lectures10
A-W-5Study of recommended literature10
60

Metody nauczania / narzędzia dydaktyczne

KODMetoda nauczania / narzędzie dydaktyczne
M-1Informative lectures with the use of multimedia presentation
M-2Laboratory works

Sposoby oceny

KODSposób oceny
S-1Ocena formująca: written test
S-2Ocena formująca: Assesment of student activity and preparing for classes

Zamierzone efekty uczenia się - wiedza

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
BTap_2A_BTA-A-D1_W01
Student has a basic knowledge in the frame of material program. Student can enurate and describe commonly used microscopic techniques.
BTap_2A_W01C-1, C-2T-W-1, T-W-2, T-W-8, T-W-3, T-W-7, T-W-5, T-W-6, T-W-4M-1S-1

Zamierzone efekty uczenia się - umiejętności

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
BTap_2A_BTA-A-D1_U01
Student is able to use commonly know microscopic techniques
BTap_2A_U08C-1, C-2T-L-5, T-L-6, T-L-1, T-L-2, T-L-7, T-L-3, T-L-4M-2S-2

Zamierzone efekty uczenia się - inne kompetencje społeczne i personalne

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
BTap_2A_BTA-A-D1_K01
Student ia aware that there is a number microscopic methods to analyse structure of living organism.
BTap_2A_K05C-1T-L-5, T-L-6, T-L-1, T-L-2, T-L-7, T-L-3, T-L-4, T-W-1, T-W-2, T-W-8, T-W-3, T-W-7, T-W-5, T-W-6, T-W-4M-1, M-2S-2

Kryterium oceny - wiedza

Efekt uczenia sięOcenaKryterium oceny
BTap_2A_BTA-A-D1_W01
Student has a basic knowledge in the frame of material program. Student can enurate and describe commonly used microscopic techniques.
2,0
3,0Student has a basic knowledge in the frame of material program
3,5
4,0
4,5
5,0

Kryterium oceny - umiejętności

Efekt uczenia sięOcenaKryterium oceny
BTap_2A_BTA-A-D1_U01
Student is able to use commonly know microscopic techniques
2,0
3,0Student with the help of teacher is able to use commonlly know microscopic techniques
3,5
4,0
4,5
5,0

Kryterium oceny - inne kompetencje społeczne i personalne

Efekt uczenia sięOcenaKryterium oceny
BTap_2A_BTA-A-D1_K01
Student ia aware that there is a number microscopic methods to analyse structure of living organism.
2,0
3,0Student express moderate interest in participating in verbal disccusion with the teacher and classmates during classes
3,5
4,0
4,5
5,0

Literatura podstawowa

  1. BRUCKER, MICROX-RAY FLUORESCENCE SPECTROSCOPY, John Wiley & Sons Ltd, 2015
  2. M. A. Nasser Hajibagheri, Electron Microscopy Methods and Protocols; Methods in Molecular Biology,VOLUME 117, HUMANA PRESS, 2011
  3. William Croft, Under the microscope A brief history of microscopy, World Scientific Publishing Co. Pte. Ltd., 2006

Treści programowe - laboratoria

KODTreść programowaGodziny
T-L-1Drying at a critical point of CO2, vacuum spraying of samples using in SEM, observations in SEM, interpretation of SE and BSE images.3
T-L-2Optical microscopy. Metallographic and biologic microscope. Observations in the light and dark field; in polarized light and using interference contrast.3
T-L-3X-ray EDS microanalysis.2
T-L-4Atomic force microscope AFM2
T-L-5The hematoxylin and eosine procedures (H&E). Application of H&E stain. Staining of tissue section with the use of H&E technique.3
T-L-6Histochemical techniques of tissue staining. Application of periodic acid Schiff (PAS) stain. Staining of renal tissue wit the use of PAS technique.3
T-L-7Immunohistechemistry (IHC). Application of IHC. Antigen retrieval. Permabilization. Blocking. Primary antibody selection and optimization. Antibody specifity. Chromogenic detection. IHC controls. Immunolocalization of aquaporin 1 (AQP1) in the renal cortex and medulla4
20

Treści programowe - wykłady

KODTreść programowaGodziny
T-W-1Basics of light optics. Construction of an optical microscope. Types of microscopic techniques in modern biology.3
T-W-2Basics of electron optics.1
T-W-3Scanning electron microscope SEM. Principle of working, construction of the microscope. Types of images. Preparation.3
T-W-4Transmission electron microscope TEM. Principle of working, construction of the microscope. Preparation.3
T-W-5X-ray microanalysis EDS and WDS.2
T-W-6Scanning Probe Microscope SPM: Scanning Tunelling Microscope STM; Atomic Force Microscope AFM.2
T-W-7Collection and preparation of biological samples for microscopic examination. Fixation, dehydratation, embedding and sectioning. Tissue preservation. Parraffin-embedded tissue. Frozen tissue. Microtome. Crystostat.2
T-W-8Tissue staining. Histological techniques (hematoxylin and eosin (H&E), periodic acid Schiff (PAS), Masson’s trichome stain, van Gison’s stain. Immunohistochemistry application. Analysis and interpretation of microscopic images4
20

Formy aktywności - laboratoria

KODForma aktywnościGodziny
A-L-1Mandatory participation in laboratories20
A-L-2Study of recommended literature10
A-L-3Preparing for the laboratory analysis20
A-L-4Consultation with the teacher10
60
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta

Formy aktywności - wykłady

KODForma aktywnościGodziny
A-W-1Participation in lectures20
A-W-2Learning for the colloquium10
A-W-3Consultation with the teacher10
A-W-4Self study content of the lectures10
A-W-5Study of recommended literature10
60
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBTap_2A_BTA-A-D1_W01Student has a basic knowledge in the frame of material program. Student can enurate and describe commonly used microscopic techniques.
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_W01has an extended knowledge in the field of biology, chemistry, mathematics, physics and related sciences adapted to the biotechnology field
Cel przedmiotuC-1Get knowledge about modern microscopy
C-2To familiarise students with the microscopy techniques and equipment used in the study of living organisms structure
Treści programoweT-W-1Basics of light optics. Construction of an optical microscope. Types of microscopic techniques in modern biology.
T-W-2Basics of electron optics.
T-W-8Tissue staining. Histological techniques (hematoxylin and eosin (H&E), periodic acid Schiff (PAS), Masson’s trichome stain, van Gison’s stain. Immunohistochemistry application. Analysis and interpretation of microscopic images
T-W-3Scanning electron microscope SEM. Principle of working, construction of the microscope. Types of images. Preparation.
T-W-7Collection and preparation of biological samples for microscopic examination. Fixation, dehydratation, embedding and sectioning. Tissue preservation. Parraffin-embedded tissue. Frozen tissue. Microtome. Crystostat.
T-W-5X-ray microanalysis EDS and WDS.
T-W-6Scanning Probe Microscope SPM: Scanning Tunelling Microscope STM; Atomic Force Microscope AFM.
T-W-4Transmission electron microscope TEM. Principle of working, construction of the microscope. Preparation.
Metody nauczaniaM-1Informative lectures with the use of multimedia presentation
Sposób ocenyS-1Ocena formująca: written test
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Student has a basic knowledge in the frame of material program
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBTap_2A_BTA-A-D1_U01Student is able to use commonly know microscopic techniques
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_U08selects and applies advanced techniques and research tools used in biotechnology
Cel przedmiotuC-1Get knowledge about modern microscopy
C-2To familiarise students with the microscopy techniques and equipment used in the study of living organisms structure
Treści programoweT-L-5The hematoxylin and eosine procedures (H&E). Application of H&E stain. Staining of tissue section with the use of H&E technique.
T-L-6Histochemical techniques of tissue staining. Application of periodic acid Schiff (PAS) stain. Staining of renal tissue wit the use of PAS technique.
T-L-1Drying at a critical point of CO2, vacuum spraying of samples using in SEM, observations in SEM, interpretation of SE and BSE images.
T-L-2Optical microscopy. Metallographic and biologic microscope. Observations in the light and dark field; in polarized light and using interference contrast.
T-L-7Immunohistechemistry (IHC). Application of IHC. Antigen retrieval. Permabilization. Blocking. Primary antibody selection and optimization. Antibody specifity. Chromogenic detection. IHC controls. Immunolocalization of aquaporin 1 (AQP1) in the renal cortex and medulla
T-L-3X-ray EDS microanalysis.
T-L-4Atomic force microscope AFM
Metody nauczaniaM-2Laboratory works
Sposób ocenyS-2Ocena formująca: Assesment of student activity and preparing for classes
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Student with the help of teacher is able to use commonlly know microscopic techniques
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięBTap_2A_BTA-A-D1_K01Student ia aware that there is a number microscopic methods to analyse structure of living organism.
Odniesienie do efektów kształcenia dla kierunku studiówBTap_2A_K05shows discipline in individual work; willing to participate in group work; can creatively plan and implement own and team activities
Cel przedmiotuC-1Get knowledge about modern microscopy
Treści programoweT-L-5The hematoxylin and eosine procedures (H&E). Application of H&E stain. Staining of tissue section with the use of H&E technique.
T-L-6Histochemical techniques of tissue staining. Application of periodic acid Schiff (PAS) stain. Staining of renal tissue wit the use of PAS technique.
T-L-1Drying at a critical point of CO2, vacuum spraying of samples using in SEM, observations in SEM, interpretation of SE and BSE images.
T-L-2Optical microscopy. Metallographic and biologic microscope. Observations in the light and dark field; in polarized light and using interference contrast.
T-L-7Immunohistechemistry (IHC). Application of IHC. Antigen retrieval. Permabilization. Blocking. Primary antibody selection and optimization. Antibody specifity. Chromogenic detection. IHC controls. Immunolocalization of aquaporin 1 (AQP1) in the renal cortex and medulla
T-L-3X-ray EDS microanalysis.
T-L-4Atomic force microscope AFM
T-W-1Basics of light optics. Construction of an optical microscope. Types of microscopic techniques in modern biology.
T-W-2Basics of electron optics.
T-W-8Tissue staining. Histological techniques (hematoxylin and eosin (H&E), periodic acid Schiff (PAS), Masson’s trichome stain, van Gison’s stain. Immunohistochemistry application. Analysis and interpretation of microscopic images
T-W-3Scanning electron microscope SEM. Principle of working, construction of the microscope. Types of images. Preparation.
T-W-7Collection and preparation of biological samples for microscopic examination. Fixation, dehydratation, embedding and sectioning. Tissue preservation. Parraffin-embedded tissue. Frozen tissue. Microtome. Crystostat.
T-W-5X-ray microanalysis EDS and WDS.
T-W-6Scanning Probe Microscope SPM: Scanning Tunelling Microscope STM; Atomic Force Microscope AFM.
T-W-4Transmission electron microscope TEM. Principle of working, construction of the microscope. Preparation.
Metody nauczaniaM-1Informative lectures with the use of multimedia presentation
M-2Laboratory works
Sposób ocenyS-2Ocena formująca: Assesment of student activity and preparing for classes
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Student express moderate interest in participating in verbal disccusion with the teacher and classmates during classes
3,5
4,0
4,5
5,0